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The Effect of Silver and Titanium Dioxide Nanoparticles on Klebsiella Pneumoniae Isolates Multi Resistant to Antibiotics and Observed by Scanning Electron Microscopy

Author: Payman A. Kareem
Dept. of Food Technology/ College of Agriculture, College of Sciences
Salahaddin University-Erbil/Iraq

Author: Essra Gh. Alsammak
Dept. of Biology/ College of Sciences Mosul University-Mosul/Iraq


DOI:
http://dx.doi.org/10.24086/cuesj.si.2017.n2a26

Abstract

One hundred fifty six samples collected included: urinary tract infections, wounds, pus, burns and tonsils from patients coming to Rizgary Teaching Hospital and Rozhawa Hospital in Erbil city from March to September 2013, scrubbed and confirmed the diagnosis (33) strain which belongs to Klebsiella pneumoniae and by (21.2%) based on cultural characteristics, microscopically features and biochemical tests in addition to the API -20E.These strains sensitivity to 12 types of antibiotics. It gave the species a high resistance against the Ampicillin (AM / 10μg) by 100% and resistant to Amoxicillin (AX / 25μg)by (94%) were less resistant to Cephalothin (KF/30µg),Ceftriaxone (CRO/30µg), Cefotaxime(CTX/30µg) by (36.4,30.3,27.3%) respectively Ten isolates were selected according to their pattern of the highest resistance as these showing multi-drug resistances and tested to specify their minimum inhibitory concentration (MIC) for the antibiotics and two types of Nanoparticles include Silver in different sizes (20, 90)nm and titanium dioxide in different sizes (10, 50, 100)nm. The results showed that the MIC for Ag 20nm was between (650 -2600) μg/ml and the MIC for Ag 90nm was between (325 -2600) μg/ml but the MIC for TiO210, 100nm between (325-2600) μg/ml, MIC of TiO250nm between (81.25-2600) μg/ml. Synergism effect between the antibiotics and the Nanoparticles when they integrate increased their effect of Klebsiella pneumoniae. Morphological changes of bacteria found using scanning electron microscope (SEM) when treating with Nanoparticles. While there a pressure on the bacterial cell surface with losing of bacterial compound.

Keywords: Klebsiella pneumoniae; multi-resistant to antibiotics; silver nanoparticles; titanium dioxide and scanning electron microscopy.

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