Molecular and Immunological Study of Hepatitis B virus infection in Samara City, Iraq.

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Author: Gailan Abdulrazaq
Directorate general of education, Salahaldden governorate
Author: Adnan F. AL-Azaawie
College of Science,  University of Tikrit

DOI: http://dx.doi.org/10.24086/cuesj.si.2017.n2a9

Abstract

Hepatitis B virus (HBV) infection is a major public health problem worldwide. The reported studies suggest an association between genotypes and disease severity. Therefore, aim of this study was to determine hepatitis B virus genotypes in chronic hepatitis B virus infection in Samara City. Blood samples from 96 patients (males (53%), females (47%) with HBV chronic infection were collected from Samarra General Hospital and blood bank in Samarra city, who diagnosed as cases of hepatitis B and attend to the hospital for monitoring and follow-up. All the serum samples were tested for HBsAg, HBsAb, HBeAg, HBeAb, HBcIgM, and HBcIgG by commercially – available (ELISA) kits, DNA was isolated from serum samples and genotypes determined by PCR technique. The Genotype D was the predominant (93.1%, 81/87) genotype in our tested samples. HB genotype C detected in 3.45 % (3/87) of tested samples. HB genotype C & F, A & D, and B & D were detected in 1.15% (1/87) for each.  Mean serum IL-17 value was lower in cases with negative viral load (2.56 ± 1.23) as determined by PCR, than all other cases with D and with mixed B and D genotypes. However, ANOVA test did not show a significance. Mean serum IL-17 was 7.94 ±5.64 in cases with HB D genotype, 1.56 ±0.57 was with genotype C, 3.25 with B & D genotype, 2.44 with A & D genotype and 1.82 with C & F genotype. The difference in mean serum value (IL-17) between negative cases and with D genotype was marginally significant, but it was significant between D genotype and combined genotypes (P=0.0018-0.0001). Conclude that HBV D genotype was the predominant, in addition, A, B, C, and F genotypes are reported for the first time in Iraq and may be Arab countries and Mediterranean Region.

Keywords: HBV, Hepatitis, Genotype. Duplicate null

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