Cihan University-Erbil Scientific Journal a periodic multidisciplinary scientific journal issued by Cihan University- Erbil after auditing and revising by a specialized staff headed by the President of the university. The journal publishes original creative researches related to all fields of pure and applied sciences and humanities in Kurdish, Arabic and English.
Related Articles
Author: Jabbar Salman Hasan
Department of Microbiology, Faculty of Medicine, Alnahrain University
Author:Ahmed Hasan Mohammed
Pathological Analysis, College of Science, Thi-Qar University
Author: Basim Mohammed Hanon
College of Veterinary Medicine, Wasit University
DOI: http://dx.doi.org/10.24086/cuesj.si.2017.n2a2
Abstract
Investigate the existence of microsatellite instability (MSI) in patients with invasive ductal breast carcinoma (IDC). Investigate the existence of microsatellite instability (MSI) in breast cancer of patients. DNA had been extracted from frozen tissue samples of breast cancer. This protocol done according to the kit manufacture’s manual of QIAamp DNA Mini kit from Qiagen – USA. All samples tested for MSI by singleplex PCR reactions using two microsatellite markers BAT 25 and BAT 26. PCR achieved in a final volume of 50 µl and after thermal cycles, gel visualization performed. PCR demonstrating microsatellite instability in 13 (26%) of the 50 breast cancer sample. Eight (16%) of 50 breast cancer sample were BAT 25 positive with a PCR product size of 124 bp, while 5 (10%) of 50 breast cancer sample were BAT 26 positive with a PCR product size 121bp. The result suggests strong evidence that microsatellite instability (MSI) at the BAT 25 and BAT 26 and have involved in the pathogenesis of the great majority of breast cancers.
Keywords: Breast Cancer, Microsatellite Instability, PCR
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