Cytogenetic Studies on Lymphocytes Isolated from Women with a History of Abortion Infected with Toxoplasma gondii

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Author: Ameena S. M. Juma
Biology Department, Cihan University-Erbil

Author: Ahmed H. Mohammed
Pathological Analysis Department, College of Science, Thi-Qar University

Author: Tarik I. AL-Jeboori
Microbiology Department, College of Medicine, Al-Nahrain University

DOI: http://dx.doi.org/10.24086/cuesj.si.2017.n2a1

Abstract

Sixty women who had abortion were selected for this study. ELISA test was used to detect anti-Toxoplasma specific antibodies. Venous heparinized blood and serum was collected from the women. Those women were divided into three groups according to the presence or absence of specific anti-Toxoplasma antibodies. These groups included women with IgG (18), women with IgM (14) and women with both IgG and IgM (12). The other 16 women had no antibodies against Toxoplasma. Twenty-four healthy-looking women had been selected as controls. Venous heparinized blood and serum was collected from those women. Serum was tested for anti-Toxoplasma antibodies. Those that revealed any antibody titer against Toxoplasma were excluded from the study. The M.I. and R.I. were found to be significantly lower in the patients when compared to the healthy controls, whereas S.C.E. was found to be significantly higher. This may indicate the effect of the parasite on the immune cells and their genetic material making it possible for the parasite to become established.

Keywords: Cytogenetic analysis, Toxoplasmosis, Abortion

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