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Quantitation assay of hepatitis C virus RNA using real-time PCR technique

Authors : Aza Bahadeen Taha, Katan Sabir Ali
Hawler Medical University,  Cihan University-Erbil

Authors : Katan Sabir Ali
Hawler Medical University,

Author: Furat Tahseen Sabeer
Public Health Laboratory,


DOI: 
http://dx.doi.org/10.24086/cuesj.si.2017.n2a22

Abstract

Hepatitis C virus is a major cause of acute and chronic infections leading to fibrosis, cirrhosis, and hepatocellular carcinoma. A real-time PCR analysis has been employed successfully for both basic research and clinical applications. The ability to monitor the real-time progress of the amplification greatly improves the PCR-based quantitation of Hepatitis C virus RNA. The study was determine the viral load of hepatitis C virus RNA by real-time PCR technique and the influence of the gender on HCV viral load. The presence of HCV antibodies were assessed in clinically suspected HCV infection patients using commercial HCV Ab ELISA test kit, then all positive test were tested for detection and quantitative of HCV RNA by real-time PCR at Public Health Laboratory in Erbil, Iraq. Among 445 patients of HCV sero positive with ELISA screening test, RNA HCV was detected in 195(43.82%) patients by quantitative real-time PCR. Based on the distribution of viral load, 38.97% of patients had viral loads of 106 IU/ml, 0.51% had viral loads between 108 and 109 IU/mL, and 6.67% had 103IU/ml. In conclusion, the highest rate of HCV was detected among sero positive males.

Keywords: HCV; antibody; real- time PCR; ELISA; quantitative assay

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