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The Protective Effect of Omega-3 Oil against Cadmium Chloride Induced Nephrotoxicity in Rat

Author: Treefa F. Ismail
College of Education, Biology Dept., Salahaddin University

Author: Falah M. Aziz
College of Science, Biology Dept., Salahaddin University

DOI: http://dx.doi.org/10.24086/cuesj.si.2017.n2a15

Abstract

The present study was designed to investigate the effect of cadmium (Cd), as cadmium chloride (CdCl2), on the kidney of male rats as well as the protective role of omega-3 oil against the Cd-induced histological, ultrastructural and immunohistochemical alterations. Thirty adult male rats were used in the present work, divided randomly into five groups, six rats in each. The first group was considered as control group and left without treatment except the standard rat chow and tap water. The second group was given (40 mg/ L) of CdCl2 in drinking water. The third group was given (60 mg/ L) of CdCl2 in drinking water. The fourth group was given (40 mg/L) of CdCl2 in drinking water plus omega-3 oil (4 gm/ kg diet) and the fifth group was given (60 mg/L) of CdCl2 in drinking water plus omega-3 oil (4 gm/ kg diet). All the above groups were left for 30days. Paraffin and plastic embedded methods were employed for the light microscopy study of the kidney in all treated animals and Immunohistochemical technique was applied to demonstrate apoptosis indicator protein (P53).Transmission electron microscopy preparations was employed for detection of further ultrastructural results. Kidney of CdCl2 treated groups has showed many histological change. These changes included dilation of the kidney tubules, degeneration of the cortical kidney tubule cells and the appearance of inflammatory leucocytes. Such changes have not been seen in the omega-3 plus both doses of CdCl2 groups. The transmission electron microscopy showed many ultrastructural changes, some of them were dilation of the vessels, highly affected podocytes, thickening of the basal lamina of the blood capillaries, lipid accumulation, degeneration of the microvilli of some proximal convoluted tubule cells and disruption of mitochondrial cristae.

Keywords: Cadmium chloride, Omega-3, Nephrootoxicity, ultrastructural and imunohistochemical.

 

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